Recombinant Human LIF

Catalog Number : L001
Available Size : 10µg, 100µg, 500µg, 1mg
Synonyms : Leukemia Inhibitory Factor, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor (MLPLI)
Description : LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Human LIF is a 19.6 kDa protein containing 180 amino acids residues, including three disulfide bonds.
Source : INNOkine recombinant human LIF is produced in rice suspension cells (Oryza sativa L. cv. Dongjin)
Purity : Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin Level : <0.05 EU/µg
Biological Activity : Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
Cross Reactivity : Mouse, Human
Research Interests : Diabetes / Weight Regulation, Immune System, Lipid Metabolism, Stem Cells & Differentiation
Research Use Only. Not for use in diagnostic or therapeutic procedures.